Preparation of biomolecules
  • cloning & mutagenesis
  • overexpression of recombinant proteins
  • purification of recombinant proteins
  • in vitro transcription of RNA
  • characterization of biomolecules
  • isotope labelling of biomolecules
  • optimization of protein solubility
cloning & mutagenesis
Vectors: pGEX-4, -6, pET-11, -15, -16, -19, -32, -35, StreptagII-vectors, modified constructs ...   
  • Thermal Cycler GeneAmp 2400 (Perkin-Elmer)
  • Thermal Cycler GeneAmp 9600 (Perkin-Elmer)
  • HeraSafe bench (Heraeus)
  • Micropulse Electroporator (Biorad)
Strains: E.c. BL21, E.c. BL21(DE3), E.c. Codon+RIL/RP (DE3), E.c. Origami, E.c. Tuner, E.c. BL21 (DE3)/pLysS ... overexpression of recombinant proteins
  
  • Fermenter Typ L1523 (BioEngineering)
  • Certomat H/HK shakers (Braun)
  • Varioklav, Autoklav 23
purification of recombinant proteins SEC (Superdex 75, Superdex 200, Fast Desalting), HIC (Phenyl-, Octyl-, Butyl-Sepharose ), RP (C4, C18), Affinity (metal chelating, GSTtrap, heparin), IEX (MonoQ, MonoS, CM-, DEAE-Sepharose, AG 1-X2), CF (MonoP) ...
  • ÄKTApurifier10 (Pharmacia Biotech)
  • Kontron HPLC (Kontron Instruments)
  • HPLC pumps 420
  • Diode Array Detector 440
  • Detector 420
  • Centrikon T-124 (Kontron)
  • Centrikon T-1170 (Kontron)
  • Christ Alpha freeze-dryer (Christ)
 ÄKTApurifier10
in vitro transcription of RNA
  • CD spectrometer Jasco J-810 with fluorescence detector (Jasco)
  • Uvicon 930 (Kontron Instruments)
characterization of biomolecules
  • Gel Doc 200 (Biorad)
  • MALDI TOF-MS (Kratos Analytical)
  • Microplate Reader (Biorad)
  • 15NH4Cl
  • 13C-Glucose
  • 2H
  • 15N, 13C-rNTPS
  • 113Cd
isotope labelling of biomolecules
optimization of protein solubility HSQC from a "biologists" view ...

... to the "NMR-spectroscopist" view