NMR spectroscopy of biomolecules


  • multidimensional NMR spectroscopy
  • NMR data analysis
  • software development

 


The spectrometers are located in a newly constructed wooden building that has been optimized for operating high field spectrometers up to 900 MHz. In particular, magentic and metallic materials were avoided in the course of construction and, special vibration damping and heavy insulation were used. The NMR-facility has a sophisticated air conditioning system that allows to maintain stable temperature and humidity at all times. Further, pure and dry pressured air and pressured nitrogen gas are produced on-site. Backup systems for air, nitrogen, and power are available to ensure continuous operation of the spectrometers. Pictures of the new NMR-facility are available here. The spectrometer laboratory has recently been approved with security level S2 status (600 MHz, 700 MHz, 800 MHz).



NMR-Spectrometers available at the Lehrstuhl Biopolymere at the Universtity of Bayreuth

Bruker AV 800 MHz, Ultrastabilized:

  • 5 mm TXI-ATM-z-Gradient probe
  • planned: 5 mm TXI-z-Gradient CryoProbe
  • 4 frequency channels
  • 100 W dedicated proton-amplifier
  • 2x 500 W broadband amplifiers
  • 4 preamplifier modules (1H, 2H, 2x X)
  • z-axis gradient amplifier
  • 20 W dedicated deuterium amplifier on 2HTX board
  • BVT 3000 and BTU 05 temperature control


multidimensional heteronuclear NMR spectroscopy:

 


elucidation of structure and dynamic properties of polypeptides, nucleic acids and their complexes

 


ligand binding studies
structure - activity studies
Bruker AV 700 MHz, Ultrashield
  • 5 mm TXI-ATM-z-Gradient probe
  • planned: 5 mm TXI-z-Gradient CryoProbe
  • 4 frequency channels
  • 100 W dedicated proton-amplifier
  • 500 W broadband amplifier
  • 300 W broadband amplifier
  • 4 preamplifier modules (1H, 2H, 2x X)
  • z-axis gradient amplifier
  • 20 W dedicated deuterium amplifier on 2HTX board
  • BVT 3000 and BTU  05 temperature control
Bruker AV 600 MHz
Bruker Avance DRX 600 MHz
  • 5 mm TXI-ATM-x,y,z-Gradient probe
  • 5 mm QXI-xyz-Gradient probe
  • 8 mm TXI-z-Gradient probe
  • 4 frequency channels
  • 50 W / 300 W combination proton / broadband amplifier
  • 2x 300 W broadband amplifiers
  • 3 preamplifier modules (1H, 2H, X)
  • xyz-axis gradient amplifier
  • 20 W dedicated deuterium amplifier on 2HTX board
  • BVT 3000 and BTU 05 temperature control
Bruker AV 400 MHz micro-console
  • 5 mm TXI-z-Gradient probe
  • 5 mm TXI-Gradient probe
  • 5 mm broadband-Gradient probe
  • 3 frequency channels
  • 50 W / 150 W combination proton / broadband amplifier
  • 300 W broadband amplifiers
  • 3 preamplifier modules (1H, 2H, X)
  • z-axis gradient amplifier
  • BVT 2000 and BTU 05 temperature control
The Chair of Biopolymers further has access to the
750 MHz and the 900 MHz NMR-spectrometer
at the Bavarian NMR-centre at TUM in Munich.



NMR data analysis


spectral processing,
assignment of spectra,
evaluation of structure and dynamic properties of biomolocules
  • NDee
  • XwinNMR
  • NMRPipe
  • NMRView
  • XPLOR
  • in-house written software